CRG/UPF Flow Cytometry Unit
CRG/UPF Flow Cytometry Unit
Overview
Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest.
The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows.
The Unit currently hosts six analyzers and five sorters. The sorters are operated by the facility personnel, who also support the users in experimental design and data analysis. The analyzers are operated by the users themselves, after they have been trained. The facility personnel help with the experimental design and supervises and monitors the experiments when necessary.
For further information, please contact Òscar Fornas.
You can learn more about the unit by taking the virtual tour HERE.
Latest Updates
Cytek® Biosciences Signs Agreement with CRG and UPF to Accelerate Scientific Discoveries (18/01/2024)
Cytek Biosciences, Inc. (Nasdaq: CTKB), a leading cell analysis solutions company, has signed an agreement with the Centre for Genomic Regulation (CRG) and the Pompeu Fabra University (UPF) to drive technological innovation and accelerate discoveries for the scientific community.
New technology acquisition:
Cytek Aurora Spectral analyzer (Cytek Biosciences)
https://cytekbio.com/pages/aurora
BigFoot Spectral Cell Sorter (ThermoFisher Scientific)
https://www.thermofisher.com/es/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/bigfoot-spectral-cell-sorter.html
BD FACSDiscover S8 Cell Sorter (Becton Disckinson)
https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-sorters/bd-facsdiscover-s8
Implemented applications:
Flow Karyotyping for chromosome sorting was implemented in our facility to isolate chromosomes for chromosome sequencing. Additionally, this methodology allows the detection of numerical and structural chromosome aberrations. Chromosomes of a single type can be purified facilitating gene mapping or production of chromosome-specific recombinant DNA libraries.
Reference: https://www.nature.com/articles/s41467-018-07885-5
Single Virus Sorting has been developed in our facility to isolate single virus. Single virus isolation allows the access to genome of single virus for sequencing. Single Virus Genomics enable the discovery of some of the likely most abundant and ecologically relevant marine viral species. The method could be used to study other biological entities into different ecosystems.
Reference: https://www.nature.com/articles/ncomms15892
Pioneering technique to discover viruses present in saliva (22/03/2018)
A group of researchers led by Manuel Martínez García, member of the University of Alicante Department of Physiology, Genetics and Microbiology, with the participation of Oscar Fornas, unit leader at the UPF-CRG Flow Cytometry Unit, has discovered new viruses present in human saliva through the application of techniques that combine flow cytometry, genomics and molecular biology.
Reference:
Cruz MJ, Martinez-Hernandez F, Garcia-Heredia I, Lluesma M, Fornas O and Martinez-Garcia M.
"Deciphering the Human Virome with Single-Virus Genomics and Metagenomics."
Viruses, March 2018.
Òscar Fornas CV
1996 PhD in Biochemistry at the Autonomous University of Barcelona (UAB), Barcelona, Spain.
1996-2000 Postdoc at Oncologic Research Institute (IRO), Barcelona, Spain.
2000-2001 PI at IDIBAPS, Barcelona, Spain.
2001-2010 Head of Flow Cytometry Core Facility at Pompeu Fabra University (UPF), Barcelona, Spain.
Since March 2010 Head of Flow Cytometry Core Facility at UPF/CRG, Barcelona, Spain.
How to access
All service offered by the Flow Cytometry Unit are accessible by web platform AGENDO.
You can access to AGENDO in the following link: https://crg.agendoscience.com/
We recommend to discuss previously the experiments with facility staff for proper experiment design.
To contact with facility staff visit our Unit members tab.
Service Prices
Services
- Unassisted bench-top analysis available by online instrument reservation.
- Assisted bench-top analysis available by appointment.
- Extensive training on bench-top analyzers.
- Extensive training on FACSAria sorter for advanced users.
- Cell sorting, staff assisted procedure, using two different high-speed sorters.
- Support in project/assay development to perform proper Flow Cytometry.
- Support on experimental advise and design.
Equipment
| Instrument | Laser Ex line (nm) |
Parameters
FSC/SSC + FL |
Application
|
| Cytek Aurora 4L | 405, 488, 561, 640 | 3 + 48 | analyzer |
| Cytek Aurora 5L | 405, 488, 640, 355, 457, 561 | 3+64 | analyzer |
| BD LSR II | 488, 633, 355, 405 | 2+10 | analyzer |
| BD LSR II b | 488, 633, 355 | 2+8 | analyzer |
| BD LSR Fortessa | 488, 640, 405, 561 | 2+13 | analyzer |
| BD FACSAria II SORP | 488, 640, 355, 405, 561 | 2 + 18 | cell sorter |
| BD Influx | 488, 640, 355, 405, 561 | 2 + 14 | cell sorter |
| BD S8 | 405, 488, 640, 355, 457, 561 | 3 + 64 | cell sorter |
| Cytek Aurora CS | 405, 488, 640, 355, 457, 561 | 3 + 64 | cell sorter |
| Thermo Bigfoot | 405, 488, 640, 355, 455, 561, 785 | 5 + 60 | cell sorter |
Software
| Software | Platform |
Analysis
|
| DiVa | PC | On-line / Off-line |
| FlowJo | PC | Off-line |
| Spectroflo | PC | Off-line |
| FCSExpress | PC / MAC | Off-line |
Cytometers Optical Configurations
Technicians
IMIM Unit Members
UPF Unit Members
