Courses@CRG : CRISPR-CAS9 tool: From Gene to Function

The CRISPR-Cas9 technology enables researchers to construct novel experiments in a wide range of fields—from basic biomedical research to biotechnology and medicine—allowing them to edit genomes at sites of their choosing.  It is now possible to create knock-in, knock-outs and mutations of any gene in any cell line or organism with high efficiency. This approach allows the perturbation of biological systems at the gene as well as at the protein level.
In silico analyses provide a good approximation of molecular structure in relation to function, and are always used to predict and enhance the success of genome editing technologies. The combination of these approaches is often used in gene therapy as well as in synthetic biology applications.

Course objective:
To introduce the student to the field of protein engineering and the use of artificial evolution to draw out new properties.  During this course, the participants will learn advanced investigation methods in enzymology and protein science, and will also obtain a basic knowledge of the underlying principles, methods and potential of genome editing using the CRISPR/Cas9 system. Finally the participants will able to design target specific CRISPR/Cas9 reagents and be able to analyze and assess the results obtained using the CRISPR/Cas9 system. The participants will also be made aware of the upcoming developments in the field of genome editing.

Course content:
•    Theoretical overview of bioinformatic tools for the experimental design of protein and genome editing/engineering

•    Theoretical overview of the CRISPR-Cas9 system for genome editing/engineering

•    Practical hands-on experience in applying Crispr-Cas9 technologies.

Participants will:

• Learn how to design and create the CRISPR/Cas9 reagents for a specific genomic target
• Learn how to select appropriate delivery vectors and editing strategies for a given project
• Learn how to generate gene knockout or knockin modified cells
• Become familiar with the various methods for mutation detection and cell manipulation using the most current technologies.

Expert scientists in the field will give talks and hold round table discussions with the students.

Organizer:
Carlo Carolis                CRG, Spain                                                                                              

Speakers/Instructors:
Pere Puigdomènech - CRAG, Spain
Marc Guell - Wyss Institute, Harvard University         
Mihail Sarov - Max Planck Institute, Germany
Jim Sawitzke - EMBL, Italy
Christina Kiel - CRG, Spain
Rory Johnson - CRG, Spain
Laura Batlle - CRG, Spain
Katrina Broadbent - CRG, Spain
Martin Gigirey - CRG, Spain
Silvia Speroni - CRG, Spain

Registration:
Only online registration is accepted
Number of participants: 16
Registration fee: 500€

Registration fee includes participation in workshop, all necessary tools and materials to follow the course, coffee breaks and lunches.

Worldwide participants will be selected according to their CV and Motivation Letter submitted.

Registration deadline : 27th June 2016 - it will be extended until 8th July!

2 Registration Fee Waivers to help lower-income students and facilitate the participation of those coming from labs located in countries in need of scientific strengthening will be offer.

Venue:
CRG – Centre for Genomic Regulation
Dr. Aiguader 88
08003 Barcelona (Spain)
How to get to the CRG ?

Contact:
Eva Navarrete
CRG – Centre for Genomic Regulation
Phone + 34 93 316 0375
Email training@crg.eu

Supported by:
We acknowledge support from the Spanish Ministry of Economy and Competitiveness,
“Centro de Excelencia Severo Ochoa 2013-2017”, SEV 2012-0208.