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Workshop on True Image Deconvolution, Restoration, and Analysis

Workshop on True Image Deconvolution, Restoration, and AnalysisWorkshop on True Image Deconvolution, Restoration, and Analysis


13/03/2025 03/04/2025

Workshop on True Image Deconvolution, Restoration, and Analysis

Online (Zoom)

If you are interested in producing high-quality microscopy images and obtaining reliable analysis results, this workshop may be of interest to you. Topics covered will include diffraction, acquisition pitfalls, spherical aberration, photon noise, Point Spread Function, Nyquist-Shannon Sampling Rate, Image Quality Control, crosstalk, (colocalization) analysis, and deconvolution. In addition, the Huygens Software will be demo-ed. Attendees will be provided with a trial version of the Huygens Software for the workshop.

Topics that are going to be addressed:

  • Imaging pyramid of compromise - frustration
  • Diffraction/blurring
  • Deconvolution
  • Acquisition pitfalls
  • Spherical aberration
  • Photon noise
  • Point Spread Function
  • Nyquist-Shannon Sampling Rate
  • Image Quality Control
  • Crosstalk
  • Chromatic aberration
  • (Colocalization) analysis

What NOT to expect:
Image restoration can significantly improve the segmentation and analysis of images, yet if the images suffer from undersampling or clipping, we recommend users to re-image their data. Nyquist-Shannon sampling should be given a higher priority than signal to background.
 
PROGRAM

March 13th (14:30-16:30):

  • 14:30 - 14:40 Presentation on Image Restoration and Analysis – with Q&A
  • 14:40 - 14:55 Image QC and pitfalls
  • 15:00 - 15:20 Deconvolution Express/Wizard
  • 15:20 - 15:30 PSF Distiller
  • 15:30 – 15:40 Stitching
  • 15:40 – 15:45 Chromatic Aberration Correction
  • 15:45 – 15:55 Workflow Processor
  • 15:55 – 16:30 Q&A

Following the 1st session, the attendees can test the software during the trial period and come with questions at the second session.

April 3rd (14:30-16:30): 

  • Post-practice discussions (Q&A)

Dates and hour: 13th March & 3rd April 2025 (14:30-16:30h)
Instructor: Vincent Schoonderwoert from Scientific Volume Imaging (SVI)
Location: Online (Zoom)
Maximum number of participants: 150
Application deadline: 10h March 2025
Target audience: Beginners
Preparation: Accepted students will receive a trial version of the software

Registration HERE

For any information, please send an email to CRG Training and Academic office (TAO): training@crg.eu
 

PDF icon huygens_workshop_2025.pdf

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