Library preparation for next generation sequencing 2019

This course will focus on the preparation of next generation sequencing libraries for sequencing on the Illumina platform. The course will include a theoretical part about sequencing technologies and a practical part scheduled in two modules to be chosen: ChIP seq library preparation (NEBNext) and mRNA seq stranded library preparation (Illumina). The course will be taught by experienced instructors from the core facility who use these protocols on a daily basis.

The course will consist of a common theoretical part and two separate modules (mRNA seq and ChIP seq) which could be taken independently.

Instructors: Jochen Hecht, Nuria Andreu Somavilla, Maria Magdalena Monfort Roca

Schedule:

• Theoretical part (common): 21st October 2019 (11:30 – 13:00)
• Module I: mRNAseq; 22nd and 23rd of October 2019 (9:30 – 17:00)
• Module II: ChIPseq; 24th of October (9:30 – 17:00)

Where: CRG Training centre (PRBB patio) and the training wet lab (6th floor)

Maximum nº of students: 16 (participants which will have the possibility to bring their own samples (max 2).

Application deadline: 5th of September 2019