CRG Core Facility Technology Symposium: High-Content Screening and Analysis

Barcelona, 14th May 2010
Organized by the Centre de Regulació Genòmica (CRG)

Symposium overview
Initially developed for secondary drug screens in the pharma industry, high-content screening is now applied at all stages of the drug discovery pipeline and has also become an important tool for basic research. In a screen, a cellular system is perturbed, either by knocking down individual genes by RNA interference or by inhibiting specific proteins or pathways with small chemical compounds, and the biological response is analysed. In high-content screening, cells are labelled with fluorescent markers and are observed and analysed using automated microscopy and automated image analysis. The combination of automated microscopy and automated image analysis allows the measurement of a multitude of cellular parameters yielding statistically robust data. In contrast to a single-readout high-throughput assay, a high-content screening assay provides many independent read-outs in a single experiment. In this way, every perturbation can be associated with a “fingerprint” rather than a simple score, phenotypes can be described with high precision, and the mode of action of the perturbing agent can be inferred.
 
With this symposium we would like to invite you to take a glimpse of what is possible with today’s screening platforms and what type of questions can be addressed. Challenges and solutions for analysing large datasets with sophisticated image analysis tools and machine learning techniques will be discussed.

Program

10:00 Welcome

10:15 Rainer Pepperkok. (Profile)
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany

"High content screening microscopy to identify new regulators in membrane traffic"

11:00 Marino Zerial.(Profile)
Max Planck Institute of Molecular Cell Biology & Genetics (MPI-CBG), Dresden, Germany

"System analysis of endocytosis by functional genomics and quantitative multi-parametric image analysis"

11:45 Coffe Break

12:15 Daniel Gerlich.(Profile)
Swiss Federal Institute of Technology Zurich (ETH Zurich), Switzerland

"High-throughput live cell imaging to screen for mitotic regulators"

13:00 Manuel Théry. (Profile)
LPCV / iRTSV / DSV / CEA, Grenoble, France

"Mechanical equilibrium in multicellular arrangements"

Organizers
Doris Meder
Raúl Gómez (visit the High-Throughput Screening Unit)
Timo Zimmermann (visit the Advanced Light Microscopy Unit)

Venue
Auditorium
CRG - Centre for Genomic Regulation
Dr. Aiguader, 88
08003 Barcelona, Spain
www.crg.es

Registration
Participation to the symposium is free of charge and open to all PRBB staff as well as external scientists. Participants should use the link on the right framework of this page for registration.

Contact person
Katia López
CRG - Centre for Genomic Regulation
Dr. Aiguader, 88
08003 Barcelona, Spain
Tel. +34 93 316 01 61
Fax +34 93 316 00 99

Language: All lectures will be in English