COURSES@CRG: From Gene to Function: Genome Editing Tools

Today´s powerful new tools enable researchers to construct novel experiments in a wide range of fields—from basic biomedical research to biotechnology and medicine—allowing them to edit genomes at sites of their choosing.  Using most recent technologies in genome engineering, it is now possible to create knock-in, knock-outs and mutations of any gene in any cell line or organism with high efficiency. This approach allows to perturb biological systems at the gene as well as at the protein level.

In silico analyses provide a good approximation of molecular structure in relation to function, and are always used to predict and enhance the success of genome editing technologies. The combination of these approaches is often used in gene therapy as well as in synthetic biology applications.

The objective of this course is to introduce the student to the field of protein engineering and the use of artificial evolution to draw out new properties.  During this course, the participants will learn advanced investigation methods in enzymology and protein science as well as the theoretical and practical notions on genome engineering tools.

The course will provide: 

1- background knowledge on bioinformatic tools for the experimental design of protein and genome engineering

2- hands-on experience in applying TALENs & Crispr-Cas9 technologies. Participants will learn to assemble TALEN and CRISPR constructs and evaluate their nuclease activity both in vitro and in vivo.

Expert scientists in the field will give talks and hold round table discussions with the students.

Organizers:

Carlo Carolis, CRG
Elias Bechara, CRG

Speakers and Instructors:

• Adrian Gustavo Turjanski, Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
• Claudio Mussolino, Centrum für Chronische Immundefizienz, Freiburg, Germany
• Fei Ann Ran, Massachusetts Institute of Technology, Boston, USA
• Marc Guell, Harvard Medical School, Boston, USA
• Marcelo Marti, Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
• Carlo Carolis Center for Genomic Regulation, Barcelona, Spain
• Katrina Broadbent Center for Genomic Regulation, Barcelona, Spain
• Martin Gigirey Suarez Center for Genomic Regulation, Barcelona, Spain
• Silvia Speroni Center for Genomic Regulation, Barcelona, Spain

Target audience and number of participants

The course targets technicians, PhD students and postdocs from the molecular biology  fields whose projects involve use of the above cited techniques

Number of participants: 16

Registration fee: 500 Euros (includes participation in workshop, all the necessary tools to follow the course, didactic material, coffee breaks, and lunches).

Registration Opens: 1st August 2015

Registration Closes: 11th October 2015

Selected Participants are communicated: 18th October 2015

Payment has to be done before 30th October 2015

Venue

CRG
Dr. Aiguader 88
08003 Barcelona
How to get to the CRG?

Contact

Eva Navarrete
Dr. Aiguader 88
08003 Barcelona
eva dot navarrete at crg dot eu