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Courses@CRG: Quantitative Light Microscopy (QLM2024)

Courses@CRG: Quantitative Light Microscopy (QLM2024)Courses@CRG: Quantitative Light Microscopy (QLM2024)

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About

Description 

This five-day comprehensive and intensive course covers the entire spectrum of bioimaging, starting from the fundamental basics of optical microscopy, instrumentation, specimen preparation, molecular markers, right up to the most recent and revolutionary development in bioimaging and bioimage data analysis, including Artificial Intelligence (AI) based analysis. The course is designed for both novice researchers and as well as those having some previous experience with microscopy and its applications. 
The course is composed of interactive lecture series to cover the theory of optical microscopy and its different modalities and applications. During the hands-on practical sessions, participants will have the opportunity to exercise on different types of light microscopes provided by the sponsors, CRG Training & Academic office. The advanced fluorescence and super-resolution microscopy hands-on practicals will be held at the Advanced Light Microscopy Unit (ALMU). Samples will be provided by ALMU and Argolight. Participants would also be able to bring their own samples if they wished to do so, following prior arrangement with the course organizers. Following the bioimage data management and analysis block, participants will have the opportunity to practice bioimaging processing and analysis, including AI-based bioimage analysis tools. At the end of each day, participants will have the chance to put their knowledge to the test with a quiz covering the day's lessons. Prizes will be awarded to the top three scorers, adding an exciting incentive to engage fully in the learning experience.
 

Main goals of the course 

  • Understand the theoretical and practical aspects of optical microscopy 
  • Understand the different imaging modalities and its applications in biomedical research 
  • Learn about bioimage data handling, processing and analysis
  • Appreciate the significance of AI in bioimaging

The course is aimed at

The course is intended for predoctoral students, postdoctoral or biomedical researchers involved with microscopy.

Topics that are going to be addressed

  • Basic microscope optics, image formation    
  • Objectives, optical aberrations, resolution 
  • Brightfield techniques, contrast generation and enhancement 
  • Microscope anatomy    
  • Introduction to fluorescence microscopy    
  • Fluorescent dyes and proteins    
  • Sample preparation 
  • Cameras for bioimaging   
  • Detectors for bioimaging and the airyscan technology     
  • Optical sectioning and confocal microscopy  
  • Nonlinear optical microscopy 
  • Lightsheet microscopy   
  • Live-cell imaging 
  • Measuring molecular dynamics and interactions: FRAP, FLIP, FLAP, FCS, FLIM, FRET, FCCS 
  • Biological electron microscopy: techniques and applications 
  • FAIR Bioimaging, Bioimage processing and data management
  • Introduction to bioimage analysis    
  • Artificial intelligence and machine learning in Bioimaging  
  • Introduction to super-resolution Microscopy  
  • STimulated Emission Depletion microscopy (STED)  
  • Single-Molecule Localisation Microscopy (SMLM)  
  • MoleculeIntroduction to Bespoke Microscope Design
  • Microscope platforms and core imaging units 

Organizers of the course

Nadia Halidi, CRG
CRG Training & Academic Office

Dates

22nd-26th April 2024 from 9:00 to 18:00h

Deadline for applications

15th March 2024

Download poster

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Program

Day 1:  Monday 22 April 2024 - Principles of Light Microscopy 

  • Welcome to the course   
  • Basic microscope optics, image formation    
  • Objectives, optical aberrations, resolution 
  • Brightfield techniques, contrast generation and enhancement 
  • Microscope anatomy  
  • Optical microscopy practical sessions    
  • Tour of the instrumentation and applications at the Advanced Light Microscopy Unit (ALMU) and EMBL Barcelona’s Mesoscopic Imaging Facility (MIF) 

Day 2: Tuesday 23 April 2024 - Fluorescence Microscopy & Optical Sectioning 

  • Light sources for bioimaging  
  • Cameras for bioimaging    
  • Detectors for bioimaging and the airyscan technology 
  • Introduction to fluorescence microscopy    
  • Fluorescent dyes and proteins    
  • Sample preparation 
  • Optical sectioning and confocal microscopy      
  • Nonlinear optical microscopy    
  • Lightsheet microscopy 

Day 3: Wednesday 24 April 2024 -  Quantitative Bioimaging I. 

  • Live-cell imaging 
  • Measuring molecular dynamics and interactions: FRAP, FLIP, FLAP, FCS, FLIM, FRET, FCCS 
  • Biological electron microscopy: techniques and applications 
  • Fluorescence microscopy practical sessions - widefield and confocal microscopy 
  • Evening social activity 

Day 4: Thursday 25 April 2024 - Quantitative Bioimaging II. 

  • FAIR Bioimaging, bioimage processing and data management    
  • Introduction to bioimage analysis with a practical session   
  • Artificial intelligence and machine learning in bioimaging with a practical session  

Day 5: Friday 26 April 2024 - Super-Resolution Microscopy 

  • Introduction to super-resolution Microscopy and Structured illumination microscopy (SIM)
  • STimulated Emission Depletion microscopy (STED) 
  • Single-Molecule Localisation Microscopy (SMLM) 
  • Introduction to bespoke microscope design
  • Microscope platforms and core imaging units
  • Super-resolution microscopy practical sessions