Courses@CRG: Biological Super-Resolution Microscopy
Courses@CRG: Biological Super-Resolution Microscopy
In the last years, super-resolution light microscopy equipment has become more widely available for biological researchers. Most if not all of the existing approaches do however present challenges in sample preparation, image acquisition and also the subsequent analysis that need to be met for the successful execution of a biological experiment. The course will provide an introduction into super-resolution imaging for STED and localization microscopy on state of the art instrumentation and focus on the important aspects of label selection, labeling strategies, sample mounting, imaging (3D) and subsequent anaylsis of STED and dSTORM datasets.
Technologies and methods:
- Labeling considerations, imaging buffers and mounting media for super-resolution microscopy: localization microscopy and STED
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Stimulated emission depletion (STED) microscopy
- Pulsed, CW and time-gated STED imaging
- Multichannel STED imaging
- 3D STED imaging
- in vivo STED imaging
-
Localization based super-resolution microscopy methods
- Multichannel 3D direct Stochastic Optical Reconstruction Microscopy (dSTORM)
- Point Accumulation for Imaging in Nanoscale Topography (PAINT)
- Rendering and processing of super-resolution datasets
- Deconvolution of 3D STED datasets
- Rendering of point-based SR data
- Registration of image channels
- Adaptive Optics in SR microscopy
| Organizer: | ||
| Timo Zimmermann | CRG, Barcelona | |
| Speakers: | ||
| Kees Jalink | Netherlands Cancer Institute, Amsterdam | |
| Lorenzo Albertazzi | IBEC, Barcelona | |
| Magali Mondin | Institite of Biology Valrose, Nice | |
| Instructors: | ||
| Raquel García | CRG, Barcelona | |
| Arrate Mallabiabarrena | CRG, Barcelona | |
| Xavier Sanjuan | UPF, Spain | |
| Nathalie Garin | Leica Microsystems |
Registration :
Only online registration is accepted
Number of participants : max. 16
Registration fee: 400€
Registration fee includes participation in workshop, all necessary tools to follow the course, didactic material, coffee breaks and lunches.
Worldwide participants will be selected according to their CV and Motivation Letter submitted.
2 Registration Fee Waivers to help lower-income students and facilitate the participation of those coming from labs located in countries in need of scientific strengthening will be offer.
Venue:
CRG – Centre for Genomic Regulation
Dr. Aiguader 88
08003 Barcelona (Spain)
How to get to the CRG ?
Contact:
Sharon Bel
CRG – Centre for Genomic Regulation
Phone + 34 93 316 0375
Email training@crg.eu
Supported by:
We acknowledge support from the Spanish Ministry of Economy and Competitiveness,
"Centro de Excelencia Severo Ochoa 2013-2017", SEV 2012-0208.